• SMALL VESSEL WIRE MYOGRAPH SYSTEMS

Small vessel wire myograph systems

Small vessel myographic systemSmall vessel myographic system

The system shows a unique modularity with features as follows:

  • Rapid extension to 1-4 channels,
  • Removable organ bath (with 2, 5, 10, 20 mL capacity),
  • Isometric measuring sensor,
  • Two sets of vessel holders, depending on the diameter of the vessel being examined (Ø60 µm – Ø3 mm),
  • Gas controller, with a freely positioned diffuser tip,
  • Continuous temperature control as per request,
  • Covering lid against evaporation and heat loss,
  • Locking of the vessel holder to facilitate positioning of the organ,
  • The measuring unit (organ bath, manipulator, force transducer) can be unlocked and removed from the base plate,
  • Field-stimulation electrodes (as per request),
  • Temperature stabilization: circulating water bath thermostat.

System

Scheme of the systemScheme of the system

The system consists of five main units:

  1. Organ bath (flow-through/stationary) with buffer in-and outflow stubs (gravitational or pumped flow) gas controller with freely positionable diffuser tip, and field-stimulating electrodes (as per request). Vessel holders: tungsten wires or steel prods (depending on vessel lumen); vessel holder locking for positioning of the organ,
  2. In- and outlet stub of the thermostated circulating water bath (20 l, 15 l/min flow rate),
  3. Precision manipulator (0.25 mm/turn),
  4. Isometric sensor,
  5. Base plate with lock/unlock lever for the measuring unit.

 

Organ bath, measuring unit

Organ bathOrgan bath

One-module system with microscopeOne-module system with microscope

The organ bath is manufactured in four different volumes (2, 5, 10, 20 ml). The standard volume is 5 ml unless requested otherwise. Each volume is available for later order, and can be replaced in the measuring unit.

The tubes carrying gas and solution for maintaining optimal physiological environment are connected detachably to the measuring unit through connectors fixed in the base plate.
Removable design of the measuring unit greatly facilitates placement of the preparation in the vessel holder because the unit can be put on the stage of a microscope.

 

Removing the measuring unit

 

The organ bath, the tension manipulator and the sensor are built into one unit which can be removed, for the sake of easy handling, in the following way:

 Unit lockingUnit locking 1. Turn the lever locking the unit to the base plate and tube connector in 90O angle Measuring unit and baseplateMeasuring unit and baseplate
 Unit slidingUnit sliding 2. Slide out the unit along the guide rail of the base plate

 

Vessel holders, precision setting of gas supply

The tungsten wire vessel holder type ISO-12-21 is recommended for vessel preparations with an inner diameter between 0.05 mm and 0.5 mm, and length up to 3.5 mm.The choice of vessel holders is determined by the inner diameter and length of the preparation. The decision finally depends on the professional competence of the person performing the experiment. The vessel holder can be interchanged in the sleeves, within and between types.  Wire-type vessel holderWire-type vessel holder
The steel prod vessel holder type ISO-12-22 can be used also for preparations with an inner diameter between 0.8 mm and 3 mm, and length over 3.5 mm. This technical solution enables the continuous examination of larger vessels in a small volume of buffer.  Steel spike-type vessel holdersSteel spike-type vessel holders

 

Gas controller and diffuser

Gas vaporizerGas vaporizer

Gas controllerGas controller

For proper working with the preparation, it is vital to have the necessary amount and adequate quality of carbogen gas evenly distributed in the solution.
To ensure the sufficient dispersion, a bubbler and fine-tuner device has been developed. The gas coming through the pressure reducer on the gas cylinder is kept in a gas bag. From there, the gas flows in a sufficiently thick-walled tube through a cylindrical clamp and is applied into the solution via a tube-tip gas diffuser.

 

Calibration

Parts of the calibration unitParts of the calibration unit

Calibration is done by means of an independent unit which can be connected to the system.

Parts of the unit:

  • Holder with the pulley with bearing,
  • Thread with hook,
  • 1g certified weight.

 

CalibrationCalibration

Steps of the calibration:

  • Remove the precision manipulator and replace it with the pulley holder,
  • Lock the isometric sensor with the lever. Hitch the thread to the stub on the vessel holder sleeve of the sensor, then lead it over the pulley,
  • Attach the 1 g calibration weight to the hook of the thread,
  • Unlock the sensor by turning the lever back in 90O.

 


Gravitational flow
Gravitational flow

Pumped flowPumped flow

Accessories required for operation

ISO-12W Workstation

The analog amplifiers, the computer and the software constitute an autonomous, integrated unit in the workstation. This solution allows fast configuration, training and promotes concentration on the experimental work.

The following hardware-software systems are recommended to graphically display, store and analyze the measured physiological parameters:

  • SPEL ISOSYS (MDE GmbH),
  • SPEL KYMOGRAPH (MDE GmbH),
  • LabChart (AD Instruments),
  • AcqKnowledge (BIOPAC),
  • LabScribe (iWorx).

ISO-12WISO-12W

EXP-ST stimulators

Square wave stimulatorSquare wave stimulator

Field stimulation electrodeField stimulation electrode

 

 

CWB-02 Circulating water bath

Circulating water bathCirculating water bath

EXP-SG amplifiers

 EXP-SG amplifier familyEXP-SG amplifier family

Ministar peristaltic pump

Ministart peristaltic pumpMinistart peristaltic pump

Z-LITE Fiber Optic Illuminator

Fiber Optic IlluminatorFiber Optic Illuminator

Surgical tools (RATKIT or individual items)

RATKITRATKIT

PZMIV-BS Precision Stereo Zoom Microscope

Precision Stereo Zoom MicroscopePrecision Stereo Zoom Microscope

Available models

You find details by clicking on the product code.

IS-05-01 One-channel* small vessel myographic system

Code Name
  Base with taps and gas adjuster (capacity for two or four units)
  10ml organ bath with force transducer and one-plane manipulator (250µm)
EXP-SG-1 DC bridge amplifier
  Micro vessel holder clamps
  100µm wolfram filament (L=3m)
OPTIONAL  
ISO-12-61 50µm wolfram filament (L=3m)
ISO-12-63 200µm wolfram filament (L=3m)
ISO-12-64 300µm wolfram filament (L=3m)
  Precision peristaltic pump
EXP-TH-1 One-channel amplifier for measuring temperature with sensor
EXP-ST-01 One-channel non-isolated stimulator with MCU controlling unit
  Field stimulating electrodes for organ bath (1 pair)
CWB-02 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1ºC)
   
SOFT-02 MDE - SPEL ISOSYS Monitoring, measuring and analysing software (16ch) system with accessories**
SOFT-03 MDE - SPEL KYMOGRAPH Monitoring, measuring and analysing software (8ch) system with accessories**
ADI-PL ADI - Power Lab with LabChart PRO software**
iWorx-LBS iWorx – LabScribe software**
Biopac-Acq BIOPAC - AcqKnowledge**

**You need to purchase only one from the sofwares.

*The system is also available in two- (IS-05-02) or four-channel (IS-05-04) models, please, contact us for further details!

References

 Year  Author  Title  Source
2002 Haj-Yehia A, Nassar T, Kadery B, Lotan C, Da'as N, Kleinman Y. Effects of the SOD mimic nitroxide 3-carbamoyl-PROXYL on oxidative stress markers and endothelial dysfunction in streptozotocin-induced diabetic rats Exp Clin Cardiol. 2002 Fall;7(2-3):85-92.
2003 Rodríguez-Sinovas A, Bis J, Anivarro I, de la Torre J, Bayés-Genís A, Cinca J. Coronary smooth muscle reactivity to muscarinic stimulation after ischemia-reperfusion in porcine myocardial infarction J Appl Physiol (1985). 2003 Jul;95(1):81-8. Epub 2003 Mar 7.
2010 Camelia, Mihai Ana; Maria, Dinca; Luciana, Dumitriu Irina; Beatrice, Gurzu Mihaela; Mihaela, Slătineanu Simona; Georgeta, Foia Liliana; Gina, Vata Luminita; Elena, Cojocaru; Gheorghe, Petrescu Apelin involvement on angiotensin II – induced venoconstriction Fiziologia - Physiology, 2010, Vol. 20 Issue 3, p8
2010 Vata LG, Dumitriu IL, Gurzu M, Slatineanu S, Vata A, Gurzu B. Ghrelin effects on local renin angiotensin from pulmonary vessels Acta Endo (Buc) 2010, 6 (3): 295-304 doi: 10.4183/aeb.2010.295
2011


Vata LG, Plamadeala P, Foia LG, Mitu F, Gorgan L, Vata A, Dumitriu IL, Gurzu B.

Obesity independent effects of high fat diet on pulmonary arteries reactivity

Acta Endocrinologica Oct-Dec2011, Vol. 7 Issue 4, p451

2012 Sotnikova R, Bacova B, Vlkovicova J, Navarova J, Tribulova N. Sex differences in endothelial function of aged hypertriglyceridemic rats – effect of atorvastatin treatment Interdiscip Toxicol. 2012 September; 5(3): 155–158.
2013 Broskova Z, Sotnikova R, Nedelcevova J, Bagi Z. Effect of a novel stobadine derivative on isolated rat arteries Interdiscip Toxicol. 2013 Jun;6(2):63-6. doi: 10.2478/intox-2013-0011.

Media

Small vessel myogrpahic sytstems cat  Download the Small vessel myographic systems catalogue in pdf version!

 

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