• ISOLATED MUSCLE TISSUE SYSTEMS

Modular tissue bath systems

Four-channel modular tissue bath systemFour-channel modular tissue bath system

FOR SMOOTH AND STRIATED MUSCLE INVESTIGATIONS

The modular setup's features

  • Easy upgrade to 2, 4, 8 channels
  • Replaceable tissue baths (5, 10, 20 ml calibrated volume)
  • Special vertical tissue baths with small volume (1, 2 ml calibrated volume)
  • Tissue holders with built in electrodes for point or field stimulation and with gas bubbler
  • Holders for vessel ring with suitable size (from Ø0,8mm) for built in stimulating electrodes
  • Gas chamber and gas flow controller
  • One-plane self-adjusting manipulator with sensor clamp (300µm)
  • Buffer reservoire with volume of 0,4 litre and 1,5 litre according to choice per channel
  • Vibration free stand
  • External thermostation (20 litre, 15 litre/min circulation speed, 0,1ºC accuracy
  • Force/displacement sensors (SEN-03-3X from 10g up to 500g)
  • Four channel bridge amplifier (EXP-SG-4).

System

Theoretical setup

Scheme of the four-channel systemScheme of the four-channel system

In order to keep the basic physiological parameters in the system a coordinated operation is necessary. The flow chart shows the operation of the system. To keep the tissue sample alive, the optimal amount, flow and temperature is necessary which is achieved by:

  1. Buffer flow to the chambers
  2. Gas bubbling (Carbogen).
  3. Keeping the temperature stable

 

 

 

Main components

One-channel assemblyOne-channel assembly

 

 

  1. Organ chamber
  2. Buffer reservoir
  3. Circulating water bath 
  4. Carbogen cylinder

 

Scheme of the operationScheme of the operation

 

The „A” tissue bath is filled with buffer from unit „B” operated by a two-way tap. The tissue bath has two outlets. The upper outlet is an overflow to keep the calibrated volume. The lower outlet is for emptying the tissue chamber during operation or during cleaning. Calibration of the tissue chamber is based on fluid displacement in the tissue chamber by the tissue holder with 1% accuracy. (eg. in case of 10ml organ chamber: inner diameter of the organ chamber = Ø 18mm, tissue holder diameter = Ø 17,5mm). The „C” unit maintains the continuous and stable temperature. The unit keeps the flow of the water in a closed circuit providing stable temperature in the water jacket of the the tissue chamber and the buffer reservoire as well. The „D” unit supplies the system with Carbogen gas filling a gas chamber. The fluid filled reservoires and tissue chambers are supplied with gas from this gas chamber through an adjuster and a fine-pore bubbler.

Buffer reservoire, tissue bath, sensor holder, gas supplier with adjuster

Buffer reservoireBuffer reservoire

Buffer reservoire, tissue bath, sensor holder and gas supplier with adjuster are the main components of one complete unit. These are assembled on the stand as one unit, then the tubing connections is done. This structure makes the replacement of any parts easy in case of damage. Moreover, malfunction of any part in the unit does not influence the operation of other units in the system, which is a further advantage.

Tissue bath with holderTissue bath with holder

Gas supplier unit with vaporizer in a four-channel systemGas supplier unit with vaporizer in a four-channel system 

Parts of the holder

The reservoires and the tissue bathes are supplied with gas through a gas chamber. Each reservoire and tissue bath is equipped with gas adjuster and bubbler. Bubblers are immersed into the buffer and maintaine the dissolved gas concentration. For the proper function of the tissue sample a suitable gas content in the buffer is absolutely necessary.  To make a large bubble surface for effective gas exchange a gas bubbler with adjuster was developed. Gas, coming from the cylinder through a reductor, is collected in a gas regulator then is driven into the bubbler head and to the buffer through a tube with suitable wall thickness.

Parts of the system - 1Parts of the system - 1 Parts of the system - 2Parts of the system - 2

The tissue holder and tissue bath volume

The tissue bath unit comprises of two holders on the vertical bar:

  • The upper one which cannot be moved
  • The lower one which holds the tissue bath and the two-way tap for filling and can be moved
 Moveable and non-moveable holdersMoveable and non-moveable holders

The tissue holders has four functions:

  • Setting of tissue bath volume by volume displacement with 0.1% accuracy
  • Providing gas into the buffer by a built in gas bubbler
  • Providing electrical stimulation of the tissue sample by built in stimulating electrodes
  • Providing a centralized suspension of the tissue sample

The bottom shape of the tissue holders enable effective buffer change/wash out and prevents sticking the bubbles on the surface.

 Types of the tissue holdersTypes of the tissue holders

Vessel ring holderVessel ring holder

 

Vessel ring holders (from Ø0,8mm) designed for built-in stimulating electrodes

 

Tissue application, pre-tension

During the development of the tissue bath system our goal was to get the tissue sample onto the tissue holder with a possible less stress and immerse as fast as possible into the oxigenated buffer. The handy solutions serve for the harmonized functionality.

1. step: The tissue holder has to be „pulled out” by lowering the tissue bath holder after loosening its fixing bolt in the rear. Then the holder is fixed again with the bolt. One end of the tissue strip is fixed onto transducer arm, the other end is fixed onto the hook on the tissue holder.

2. step: The holder with tissue bath is slid upwords after loosening the fixing bolt until the tissue holder with the sample on it fully immersed into the carbogenized buffer.

3. step: The excess of buffer drained through the overflow providing the previously set calibrated volume in the chamber. Then the bolt on the rear is fixed again.

 Tissue applicationTissue application

Small volume tissue bathes (SD1-v0, SD2-v0)

A complementary SD tissue bath with 1ml or 2ml volume is available. These bathes are also equipped with bubbler, tissue holder, etc. like standard bathes.

1. step: Lower the tissue bath holder after loosening its fixing bolt in the rear. Remove the tissue holder and replace it with the SD bath then fix it with a screw.

2. step: Pull out the tissue holder of SD bath. Place the tissue sample on it.

3. step: Lift the tissue bath holder until the SD bath immersed into the buffer. The SD bath will be filled with the buffer. Following this lower the tissue bath again until the SD just above the buffer level in the tissue bath. Fix the organ bath holder with the bolt on the rear.

 Application of the small-volume bathesApplication of the small-volume bathes Parts of the small-volume bathParts of the small-volume bath 

Recommended software/hardware for recording

For recording and analysis of measured parameters we offer the following software/hardware systems:

  • SPEL ISOSYS (MDE GmbH),
  • SPEL KYMOGRAPH (MDE GmbH),
  • LabChart (AD Instruments),
  • AcqKnowledge (BIOPAC),
  • LabScribe (iWorx).

 

Accessories required for operation 

CWB-02 Circulating water bath

Circulating water bathCirculating water bath

 

EXP-SG-4 amplifiers

EXP-SG amplifier familyEXP-SG amplifier family

 

 EXP-ST-04 stimulators

Square wave stimulatorsSquare wave stimulators

Available models

You find details by clicking on the product code.

IS-01 Four-channel modular tissue bath system for smooth and striated muscle investigations

Code Quantity Name
  1 Stand with taps (4 pcs) and one-plane stretcher manipulators (4 pcs)
  4 Organ chambers with holders*
  4 Buffer reservoires (0.4 l/pc)
  4 Teflon tissue holders with gas vaporizer and stimulating electrode**
OPTIONAL    
EXP-SG-4 1 Four- channel DC bridge amplifier
SEN-03-3X 4 Force/displacement transducer (select the size ofthe sensor)
EXP-ST-04 1 Four-channel non-isolated stimulator with MCU controlling unit
CWB-02 1 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1°C)
ISO-08-5X 1 Vessel ring holders*** (10 pairs/pack) 
SOFT-02 1 MDE - SPEL ISOSYS Monitoring, measuring and analysing software (16ch) system with accessories****
SOFT-03 1 MDE - SPEL KYOMGRAPH Monitoring, measuring and analysing software  (8ch) system with accessories****
ADI-PL 1 ADI Power Lab with LabChart PRO software****
iWorx-LBS 1 iWorx – LabScribe software****
Biopac-Acq 1 BIOPAC - AcqKnowledge****

*Choose organ chamber volume: 5 ml, 10 ml or 20 ml.
**Choose the type of stimulation: no, point, ring, zig-zag or other.
***Choose the type from ISO-08-5X datasheet.
****You need to purchase only one from the sofwares.

IS-02 Eight-channel modular tissue bath system for smooth and striated muscle investigations

Code Quantity Name
  2 Stand with taps (4 pcs) and one-plane stretcher manipulators (4 pcs)
  8 Organ chambers with holders*
  8 Buffer reservoires (0.4 l/pc)
  8 Teflon tissue holders with gas vaporizer and stimulating electrode**
OPTIONAL    
EXP-SG-4 2 Four- channel DC bridge amplifier
SEN-03-3X 8 Force/displacement transducer (select the size ofthe sensor)
EXP-ST-04 2 Four-channel non-isolated stimulator with MCU controlling unit
CWB-02 1 Circulating water bath (20 litres, 20 l/min delivery speed, ±0,1°C)
ISO-08-5X 1 Vessel ring holders*** (10 pairs/pack) 
SOFT-02 1 MDE - SPEL ISOSYS Monitoring, measuring and analysing software (8ch) system with accessories****
SOFT-03 1 MDE - SPEL KYOMGRAPH Monitoring, measuring and analysing software (8ch) system with accessories****
ADI-PL 1 ADI Power Lab with LabChart PRO software****
iWorx-LBS 1 iWorx – LabScribe software****
Biopac-Acq 1 BIOPAC - AcqKnowledge****

*Choose organ chamber volume: 5 ml, 10 ml or 20 ml.
**Choose the type of stimulation: no, point, ring, zig-zag or other.
***Choose the type from ISO-08-5X datasheet.
****You need to purchase only one from the sofwares.

References

 Year  Author  Title  Source
2001 Ducza E, Gáspár R, Márki A, Gyula P, Bottka S, Falkay G. Use of antisense oligonucleotides to verify the role of the alpha(1A)-adrenergic receptor in the contractility of the rat uterus post partum Mol Pharmacol. 2001 May;59(5):1235-42.
2002 Klukovits A, Gáspár R, Sántha P, Jancsó G, Falkay G. Functional and histochemical characterization of a uterine adrenergic denervation process in pregnant rats Biol Reprod. 2002 Sep;67(3):1013-7.
2003 Rubovszky B, Szentmiklósi AJ, Márián T, Cseppento A, Gesztelyi R, Székely A, Fórizs F, Gáspár R, Trón L, Krasznai Z. Comparative pharmacological studies on the A2 adenosine receptor agonist 5'-n-ethyl-carboxamidoadenosine and its F19 isotope labelled derivative J Pharmacol Sci. 2003 Nov;93(3):356-63.
2003 Mihályi A, Ducza E, Gáspár R, Falkay G. Investigation of the role of the serotonergic activity of certain subtype-selective alpha1A antagonists in the relaxant effect on the pregnant rat uterus in vitro Mol Hum Reprod. 2003 Aug;9(8):475-80.
2003 Gesztelyi R, Zsuga J, Hajdú P, Szabó JZ, Cseppento A, Szentmiklósi AJ. Positive inotropic effect of the inhibition of cyclic GMP-stimulated 3',5'-cyclic nucleotide phosphodiesterase (PDE2) on guinea pig left atria in eu- and hyperthyroidism Gen Physiol Biophys. 2003 Dec;22(4):501-13.
2003 Gesztelyi R, Zsuga J, Cseppentõ A, Bajza A, Varga A, Szabó JZ, Szentmiklósi AJ. Special sensitization pattern in adenosine-induced myocardial responses after thyroxine-treatment J Pharmacol Sci. 2003 Apr;91(4):295-304
2004 Klukovits A, Gaspar R, Santha P, Jancso G, Falkay G. Role of capsaicin-sensitive nerve fibers in uterine contractility in the rat Biol Reprod. 2004 Jan;70(1):184-90. Epub 2003 Oct 1.
2005 Gáspár R, Ducza E, Mihályi A, Márki A, Kolarovszki-Sipiczki Z, Páldy E, Benyhe S, Borsodi A, Földesi I, Falkay G. Pregnancy-induced decrease in the relaxant effect of terbutaline in the late-pregnant rat myometrium: role of G-protein activation and progesterone Reproduction. 2005 Jul;130(1):113-22.
2006 Karsai D, Zsuga J, Juhász B, Dér P, Szentmiklósi AJ, Tósaki A, Gesztelyi R. Effect of nucleoside transport blockade on the interstitial adenosine level characterized by a novel method in guinea pig atria J Cardiovasc Pharmacol. 2006 Jan;47(1):103-9.
2006

Grzesk G, Radomski M, Wicinski M, Swiatkiewicz I,
Sukiennik A, Kubica J, Szadujkis-Szadurski L.

Zmiana reaktywności mięśniówki gładkiej tętnicy po ekspozycji na środki cieniujące stosowane w angiografii Cardiovascular Forum 2006, 11, 4, 135–140.
2007 Brugós L, Gesztelyi R, Zsuga J, Cseppento A, Benko I, Galajda Z, Deák G, Sipka S, Roszer T, Kovács P, Szilasi M, Edes I, Szentmiklósi AJ. Modulation of adenosine-induced responses in the guinea-pig trachea during long-term caffeine treatment: possible role of epithelium J Pharmacol Sci. 2007 Nov;105(3):279-90.
2007 Csonka D, Zupkó I, Minorics R, Márki A, Csík G, Falkay G. The effects of alpha-methyldopa on myometrial noradrenaline release and myometrial contractility in rat Acta Obstet Gynecol Scand. 2007;86(8):986-94.
2008 Ilieva GS, Tolekova AN, Sandeva RV, Trifonova KJ, Tsokeva ZI, Ganeva MG, Mihova ZM, Tolev AT, Zezovski SL. Role of transmembrane calcium current in angiotensin ii-mediated contraction of detrusor organ strips from rat urinary bladder Bulgarian Journal of Veterinary Medicine (2008), 11, No 2, 89−94.
2008

Grzesk G, Kubica A, Grzesk E, Kozinski M,
El-Essa A, Szadujkis-Szadurska K, Sukiennik A,
Swiatkiewicz I, Wicinski M.

Wpływ jonowych i niejonowych środków cieniujących stosowanych w angiografii na aktywność śródbłonka naczyniowego Folia Cardiologica Excerpta 2008, tom 3, nr 12, 586–592.
2009 Orescanin-Dusić Z, Milovanović S, Radojicić R, Nikolić-Kokić A, Appiah I, Slavić M, Cutura N, Trbojević S, Spasić M, Blagojević D. Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated Gen Physiol Biophys. 2009;28 Spec No:143-8.
2009 Appiah I, Milovanovic S, Radojicic R, Nikolic-Kokic A, Orescanin-Dusic Z, Slavic M, Trbojevic S, Skrbic R, Spasic MB, Blagojevic D. Hydrogen peroxide affects contractile activity and anti-oxidant enzymes in rat uterus Br J Pharmacol. 2009 Dec;158(8):1932-41. doi: 10.1111/j.1476-5381.2009.00490.x.
2009 Sprossmann F, Pankert P, Sausbier U, Wirth A, Zhou XB, Madlung J, Zhao H, Bucurenciu I, Jakob A, Lamkemeyer T, Neuhuber W, Offermanns S, Shipston MJ, Korth M, Nordheim A, Ruth P, Sausbier M. Inducible knockout mutagenesis reveals compensatory mechanisms elicited by constitutive BK channel deficiency in overactive murine bladder FEBS J. 2009 Mar;276(6):1680-97. doi: 10.1111/j.1742-4658.2009.06900.x. Epub 2008 Feb 12.
2009 Minorics R, Gáspár R, Gál A, Klukovits A, Falkay G. Progesterone decreases the relaxing effect of the beta3-adrenergic receptor agonist BRL 37344 in the pregnant rat myometrium Reproduction. 2009 Aug;138(2):383-90. doi: 10.1530/REP-09-0116. Epub 2009 May 21.
2010 Klukovits A, Tekes K, Gündüz Cinar O, Benyhe S, Borsodi A, Deák BH, Hajagos-Tóth J, Verli J, Falkay G, Gáspár R. Nociceptin inhibits uterine contractions in term-pregnant rats by signaling through multiple pathways Biol Reprod. 2010 Jul;83(1):36-41. doi: 10.1095/biolreprod.109.082222. Epub 2010 Mar 17.
2010 Bigovic D, Brankovic S, Kitic D, Radenkovic M, Jankovic T, Savikin K, Zivanovic S. Relaxant effect of the ethanol extract of helichrysum plicantum on isolated rat ileum contractions Molecules. 2010 May 10;15(5):3391-401. doi: 10.3390/molecules15053391.
2010 Csik G, Spiegl G, Minorics R, Falkay G, Zupko I. The effects of adjuvant arthritis on the myometrial adrenergic functions in the nonpregnant and the late-pregnant rat J Physiol Pharmacol. 2010 Oct;61(5):629-36.
2011 Christian F, Szaszák M, Friedl S, Drewianka S, Lorenz D, Goncalves A, Furkert J, Vargas C, Schmieder P, Götz F, Zühlke K, Moutty M, Göttert H, Joshi M, Reif B, Haase H, Morano I, Grossmann S, Klukovits A, Verli J, Gáspár R, Noack C, Bergmann M, Kass R, Hampel K, Kashin D, Genieser HG, Herberg FW, Willoughby D, Cooper DM, Baillie GS, Houslay MD, von Kries JP, Zimmermann B, Rosenthal W, Klussmann E. Small molecule AKAP-protein kinase A (PKA) interaction disruptors that activate PKA interfere with compartmentalized cAMP signaling in cardiac myocytes J Biol Chem. 2011 Mar 18;286(11):9079-96. doi: 10.1074/jbc.M110.160614. Epub 2010 Dec 22.
2011 Brankovic S, Kitic D, Radenkovic M, Veljkovic S, Jankovic T, Savikin K, Zdunic G. Spasmolytic activity of the ethanol extract of Sideritis raeseri spp. raeseri Boiss. & Heldr. on the isolated rat ileum contractions J Med Food. 2011 May;14(5):495-8. doi: 10.1089/jmf.2010.0036. Epub 2011 Apr 11.
2012 Hadzhibozheva PV, Georgiev TK, Kalfin RE, Tolekova AN. Angiotensin II and Vasopressin effects on motor activity of rat isolated tissue strips from urinary bladder and rectum Bulgarian Chemical Communications 01/2012; 44(3):252-257.
2012 Hadzhibozheva PV, Tolekova AN, Mihaylova S, Georgiev TK. AT2 receptors and angiotensin II-mediated contractions of gastrointestinal tract of rats Trakia Journal of Sciences, Vol. 10, Suppl. 1, 161-166, 2012
2012 Kitic D, Brankovic S, Radenkovic M, Savikin K, Zdunic G, Kocic B, Velickovic-Radovanovic R. Hypotensive, vasorelaxant and cardiodepressant activities of the ethanol extract of Sideritis raeseri spp. raeseri Boiss & Heldr J Physiol Pharmacol. 2012 Oct;63(5):531-5.
2012 Appiah I, Nikolic-Kokic A, Orescanin-Dusic Z, Radojicic R, Milovanovic S, Spasic M, Blagojevic D. Reversible oxidation of myometrial voltage-gated potassium channels with hydrogen peroxide Oxid Med Cell Longev. 2012;2012:105820. doi: 10.1155/2012/105820. Epub 2012 Oct 24.
2012 Tolekova A, Hadzhibozheva P, Georgiev T, Mihailova S, Ilieva G, Gulubova M, Leventieva-Necheva E, Milenov K, Kalfin R. The effects of some neuropeptides on Motor Activity of smooth muscle organism abdominal and pelvic cavities In Neuroscience » "Neuroendocrinology and Behavior" book edited by Tomiki Sumiyoshi, ISBN 978-953-51-0740-8, Published: September 19, 2012.
2013 Hadzhibozheva PV, Tolekova AN, Georgiev TK. Angiotension II receptors - role for the contraction of large intestine Bulgarian Journal of Veterinary Medicine 01/2013; 16(Suppl. 1):10-18.
2013 Dovinová I, Barancik M, Majzunova M, Zorad S, Gajdosechová L, Gresová L, Cacanyiova S, Kristek F, Balis P, Chan JY. Effects of PPARgamma agonist pioglitazone on redox-sensitive cellular signaling in young spntaneously hypertensive rats PPAR Res. 2013;2013:541871. doi: 10.1155/2013/541871. Epub 2013 Dec 19.
2013 Roza O, Lovász N, Zupkó I, Hohmann J, Csupor D. Sympathomimetic activity of a Hoodia gordonii product: a possible mechanism of cardiovascular side effects Biomed Res Int. 2013;2013:171059. doi: 10.1155/2013/171059. Epub 2013 Nov 6.
2013 Nikolic-Kokic A, Orescanin-Dusic Z, Slavic M, Spasojevic I, Stevic Z, Spasic M, Blagojevic D. The effects of human wild-type and fals mutant L144P SOD1 on non-vascular smooth muscle contractions Journal of Medical Biochemistry;Oct2013, Vol. 32 Issue 4, p375.
2013 Deák BH, Klukovits A, Kormányos Z, Tekes K, Ducza E, Gáspár R. Uterus-relaxing effects of nociceptin and nocistatin: Studies on preterm and term-pregnant human myometrium in vitro Reprod Sys Sexual Disorders 2:117. doi: 10.4172/2161-038X.1000117.
2014 Grześk G, Malinowski B, Grześk E, Wiciński M, Szadujkis-Szadurska K. Direct regulation of vascular smooth muscle contraction by mastoparan‑7 Biomed Rep. 2014 Jan;2(1):34-38. Epub 2013 Oct 4.
2014 Ahmed AAE, Gáspár R, Márki Á, Mudawi MME, Falkay Gy. Partial agonistic property of new isolated natural compounds Journal of Chemical and Pharmaceutical Research, 2014, 6(3):1294-1298.

Media

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